An Air France plane has been isolated at an airport in Madrid after a patient was reported to have a fever and shivers.
The situation is being treated as a suspected case of ebola, a health ministry official was quoted as saying.
There were 163 passengers on the flight from Paris, according to local officials and media reports.
Airports operator Aena and Air France said in separate statements that a passenger on Air France 1300 from Nigeria, via the French capital, had started shaking during the flight.
Air France said the other passengers got off the plane, which will now be disinfected.
The return flight has been cancelled.
Meanwhile, in Denmark, a health worker is being tested for ebola.
The European Union is to launch an "immediate" review of exit screening in African countries hit by ebola.
Spain's government has stepped up its response to suspected ebola cases in the wake of a health scare when a nurse in Madrid became the first person outside Africa to become infected with the deadly virus.
Teresa Romero, was diagnosed with the virus last week and is still seriously ill but stable. She cared for two infected priests repatriated from West Africa who later died.
A person who had been in contact with Ms Romero and was being monitored remotely for signs of the disease would be hospitalised, after developing a fever, Spanish authorities said.
The person was one of 68 considered to have a low risk of catching ebola, and who have to check their temperature regularly from home.
Another 15 people, including Ms Romero's husband, are still under observation in Madrid's Carlos III hospital where she is also being treated, but have displayed no symptoms.
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